hepatitis a virus test kits at competitive price

hepatitis a virus test kits at competive price

Packaging & Shipping&Delivery

SUMMARY AND PRINCIPLE OF THE TEST

Hepatitis B is an infectious illness caused by hepatitis B virus (HBV) which infects the liver of hominoidea, including humans, and causes an inflammationcalled hepatitis. HBV is excreted in body fluids such as semen, saliva, blood and urine in persons with acute or chronic infection. The hepatitis B virus is known as a blood-borne virus because it is transmitted from one person to another via blood or fluids contaminated with blood.

About 7.18% carry hepatitis B Virus surface antigen. About 93 million people are chronic carriers of HBV among which about 30 million are suffering Hepatitis B around the country. Transmission of hepatitis B virus results from exposure to infectious blood or body fluids.

When HBV invades the body it causes liver damage through induction of auto-immunity. Three immunity has been found, surface antigen (HBsAg)/HBsAb, core antigen (HBcAg)/HBcAb and e antigen(HBeAg)/HBeAb. As it is difficult to detect the core antigen in the serum, the other five are been done to diagnosing HBV. The surface antigen contains the determinant “a”, common to all known viral subtypes and immunologically distinguished in two distinct subgroups (ay and ad). HBV has 10 major serotypes and four HBsAg subtypes have been recognized (adw, ady, ayw, and ayr). HBsAg can be detected 2 to 4 weeks before the ALT levels become abnormal and 3 to 5 weeks before symptoms develop. The serological detection of HBsAg is a powerful method for the diagnosis and prevention of HBV infection and ELISA has become an extensively used analytical system for screening of blood donors and clinical diagnosis of HBV in infected individuals

REAGENTS

Materials provided with the kits:

 Materials required but not provided:

1. Precision pipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.

2. Disposable pipette tips.

3. Distilled water.

4. Humidified Box capable of maintaining 37 °C

5. Absorbent paper or paper towel.

6. Microtiter plate or strip-well washer

7. Microtiter plate reader with 450nm (or 450 nm/630 nm)wavelength

8. Timer

SPECIMEN COLLECTION AND PREPARATION

Serum should be prepared from a whole blood specimen obtained by acceptable medical techniques. Either serum or plasma can be used in this test.  Remove serum or plasma from the clot or blood cells as soon as possible to avoid hemolysis.  Specimen with extensive particulate should be clarified by centrifugation prior to use.  Specimen frozen at -20°C or colder may be used.  Avoid repeated freeze thaw.

PRECAUTIONS FOR USERS

1. For in-vitro diagnostic use only.

2. Do not use kit beyond expiration date.

3. Do not mix components from kits with different lot number.

4. Avoid microbial contamination of reagents.

5. Do not pipet reagent by mouth and no smoking or eating while performing assays.

6. Wear gloves during the whole process and avoid reagents or specimen spilling-out.

7. Wipe up the spills using 5% hypochlorite solution.

8. Decontaminate all liquids or solid wastes before deposing.

STORAGE OF TEST KITS

Unopened test kits should be stored at 2-8°C. DO NOT FREEZE KIT COMPONNETS.  The microtiter plate should be kept in a sealed bag to minimize exposure to damp air. Use up the reagents as soon as possible after the kit is unpacked.

ASSAY PROCEDURE:

It is strongly advised to analyze each specimen and controls in duplicate. All the reagents should equilibrate to room temperature before use.

1. Dispense one drop (100μl) of Positive Control as well as Negative Control in duplicate into respective wells. Set one blank well as background control, and 100ul of serum or plasma samples into respective test wells.

2. Place the microtiter plates into a humidified box, and incubate at 37 °C for 60 minutes.

3. Add one drop (50μl) of Enzyme Conjugate to each well. Mix it gently by swirling the microtiter plate on flat bench for 1 minute. Do not add Enzyme Conjugate to the blank well.

4. Place the microtiter plates into a humidified box, and incubate at 37 °C for 30 minutes.

5. Wash each well 5 times by filling each well with diluted wash buffer, then inverting the plate vigorously to get all water out and blocking the rim of wells on absorbent paper for a few seconds.

6. Add one drop (50μl) of Substrate Solution A (HRP-substrate) to each well, then add one drop (50μl) of Substrate Solution B (TMB) to each well. Mix gently and incubate at 37 °C

for 30minutes.

7. Add 1 drop (50μl) of Stop Solution to each well to stop the color reaction. Read the OD value at 450 nm/630 nm with dual filter plate reader. It is option to read the OD value at 450 nm with single filter plate reader. (using the OD value of the blank well to correct all the OD reading from all wells)

1. Samples: free

2. OEM services: we accept customized services