Wondfo H1N1 Influenza B Test

Wondfo One Step Influenza B Test is a rapid immunochromatographic test for the visual detection of influenza type B antigen (nucleoprotein) extracted from the nasal swab specimen. The test adopts double antibody sandwich method.

TEST PROCEDURE

Allow the test device, specimen, and extraction buffer to equilibrate to room temperature (10°C ~30°C) prior to testing.

1. Use an extraction tube (provided) for each specimen to be tested, and label each tube appropriately. 2. Hold the extraction buffer bottle upside down vertically, then add 8 drops (about 400μl) of extraction buffer to the extraction tube.

3. Place the specimen swab in the tube and swirl the swab for 10 times while pressing the swab head against the inside of the tube to release the specimen in the swab.

4. Remove the swab while squeezing the swab head against the inside of the collection tube as you remove it to expunge as much liquid as possible from the swab. Discard the swab. Cap the tube and mix contents by gently swirling. The extraction specimen must be tested immediately.

5. Remove the test device from its sealed foil pouch by tearing at the notch. Dispense 3~4 drops (80μl~100μl) of extraction specimen from the extraction tube into the sample well of the test device by inverting and squeezing the tube as shown. 6. Read the test results at 15~20 minutes. DO not read test results after 30 minutes.

INTERPRETATION OF RESULTS

Positive (+)

Colored bands are visible in both the control region and the test region. It indicates a positive result for influenza type B antigen.

Negative (-)

A colored band is visible only in the control region. No color band appears in the test region. It indicates that the concentration of the influenza type B antigen is zero or below the detection limit of the test.

Invalid

No visible band at all, or there is a visible band only in the test region but not in the control region. Repeat with a new test kit. If test still fails, please contact Wondfo or the distributor for technical assistance.

Note: There is no meaning attributed to line color intensity or width.