Marek's disease virus Antibody ELISA Kit
1. Usage
The MDV Ab ELISA is a blocking Enzyme Linked Immunosorbent Assay for the qualitative detection of specific antibodies to Newcastle disease Virus (MDV)in avian serum.
2. Principle
The MDV Ab ELISA contains a microplate, which is pre-coated with recombinant Ag on the well. For testing, ELISA plates are incubated with a serum and control(1:10 dilution with the sample diluents) for 60 minutes at 37℃. During first incubation, anti-MDV antibodies present in sample bind onto the antigen coated on the well. After incubation and washing step, then mAb-HRP is dispensed into the wells and incubated for 60 minutes at 37℃. Following this incubation, all unbounded materials are removed by washing step. The enzyme linked to the complex is revealed by addition of a substrate. The enzyme activity will thus be in inverse proportion to the anti-MDV antibodies in specimens and evidenced by incubating the solid-phase with a substrate solution. The reaction is stopped by adding stop solution, and colorimetric reading will be performed by using a spectrophotometer at 450nm and reference wavelength at 620nm.
3.The Kit components
Reagent Volume 96 Tests/192Tests
Antigen coated micro-assay plate 1ea/2ea
Negative Control 0.8ml/1.6ml
Positive Control 0.8ml/1.6ml
Sample dilute solution 50/100ml
Washing solution (10X concentrated) 50/100ml
Enzyme conjugate 6/11ml
Substrate 11/22ml
Stopping solution 6/11ml
Adhesive plate sealer 2ea/4ea
Dilution microplate 1ea/2ea
Product specification 1 pcs
Shenzhen Zhenrui Biotechnology Co., Ltd. was established in 2013 in Shenzhen. Focus on research and produce animal diagnostic test kits. Our main products is rapid test kit and Elisa test kit for companion animals and livestock.
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