Veterinary Diagnostic Assays Avian Marek's disease virus antibody ELISA kit

                                                            Marek's disease virus Antibody ELISA Kit

                1. Usage

          The MDV  Ab ELISA is a blocking Enzyme Linked Immunosorbent Assay for the qualitative detection of specific antibodies to Newcastle disease Virus (MDV)in avian  serum.

            2. Principle

 The MDV Ab ELISA contains a microplate, which is pre-coated with recombinant Ag  on the well. For testing, ELISA plates are incubated with a serum and control(1:10 dilution with the sample diluents) for 60 minutes at 37℃. During first incubation, anti-MDV antibodies present in sample bind onto the antigen coated on the well. After incubation and washing step, then mAb-HRP is dispensed into the wells and incubated for 60 minutes at 37℃. Following this incubation, all unbounded materials are removed by washing step. The enzyme linked to the complex is revealed by addition of a substrate. The enzyme activity will thus be in inverse proportion to the anti-MDV antibodies in specimens and evidenced by incubating the solid-phase with a substrate solution. The reaction is stopped by adding stop solution, and colorimetric reading will be performed by using a spectrophotometer at 450nm and reference wavelength at 620nm.

3.The Kit components

    Reagent Volume                                   96 Tests/192Tests
   Antigen coated micro-assay plate                 1ea/2ea
   Negative Control                                         0.8ml/1.6ml
   Positive Control                                           0.8ml/1.6ml
   Sample dilute solution                                      50/100ml
   Washing solution (10X concentrated)               50/100ml
   Enzyme conjugate                                               6/11ml
   Substrate                                                           11/22ml
   Stopping solution                                                 6/11ml

   Adhesive plate sealer                                       2ea/4ea
   Dilution microplate                                            1ea/2ea
   Product specification                                           1 pcs