Quick Bacteria Genomic DNA Extraction Kit/ DNA Isolation Kit 100preps N1152

   Quick Bacteria Genomic DNA Ectraction Kit

                   For research use only

Kit Content

Description

The bacteria Genomic DNA Purification Kit provides a simple and rapid method for high quality genomic DNA purification from Gram Positive and Gram Negative Bacteria. The bacteria Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-20 µg from 0.5 – 2 ml of bacteria culure. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.

Applications

Purified DNA is free from contaminants and enzyme inhibitors, and typically has A₂₆₀/A₂₈₀ ratios between 1.7 and 1.9, and is suitable for applications such as:

• Restriction digestion
• PCR
• Labeling
• Library construction

Feature

• Safe - no phenol extraction step.
• Fast - procedure takes only 30 min.
• Efficient – 3-20 µg of genomic DNA from 1.5 – 2 ml bacteria culure.
• High purity - Purified DNA is ready for downstream application such as PCR, restriction digestion.

Store

store Protein K at -20°C, other reagents can be store at room temperature for up to 1 year. Any precipitate in the Solution DS and solution MS can be re-dissolved by incubating at 37°C before use.