Plant Genomic DNA Kit for DNA extraction for Nucleic acid purification

Plant Genomic DNA Kit

—— For genomic DNA purification from powder or polysaccharide polyphenol rich plants

Cat. No:
4992201

Packing Size:
50 preps

Kit Contents:

Storage:
Room temperature (15-25℃)

Description:

The Plant Genomic DNA Kit provides a fast, simple, and cost-effective genomic DNA extraction method for routine molecular biology laboratory applications. It’s especially suitable for genomic DNA purification from powder or polysaccaride/ polyphenol rich plants. The kit is based on the silica membrane technology and a unique buffer to eliminate polysaccaride, polyphenol, and enzyme inhibitors. Plant Genomic DNA Kit is ready-to-use to purify the genomic DNA from a wide variety of plants. The purified DNA is suitable for PCR, restriction digestion and Southern blot.

Required Reagents:

Liquid nitrogen, Ethanol, Phenol-chloroform, RNaseA (optional)

Features:

• Simple and fast: The purified gDNA could be obtained within 1 hour.
• High purity and excellent quality: The high pure genomic DNA could be used directly in downstream experiments such as PCR amplification, restriction endonuclease digestion and Southern blot.

Applications:

• Suitable for various plant tissues.
• Particularly suitable for polysaccaride/polyphenol-rich plant tissues.
• Particularly suitable for plant powders.

DNA Extraction Yield From Various Plant Tissues:

Note: DNA yield depends on sample types. All the materials above are from tender leaves.

Experimental Example:

Genomic DNA isolated from 100 mg leaves of different plants using Plant Genomic DNA Kit. 3 μl of 100 μl eluates were loaded per lane.

1: Fresh tomato leaves;

2: Fresh cotton leaves;

3: Fresh tea leaves;

4: Fresh strawberry leaves.

The concentration of the agarose gel was 1%. The electrophoresis was performed under 6 V/cm for 20 min.

M: λDNA/Hind III Marker