1. Before testing, the subject shall mortify for 3-7 days, too short or too long abstinence time will affect the accuracy of diagnosis.
2. Specimen collection of semen: Using masturbation to expel the semen directly into the sperm collecting cup.
3.Liquefaction: To shake evenly the semen in the semen collecting cup, and standing it for 15 min,until the semen becomes fluid liquid.