Crystal violet will bind equally well to DNA in cell nucleus and make it colored. When it used to stain cells or tissues, the nuclei shows sharp purple and is easily differentiated in microscopy. Crystal violet staining has been widely used in cytology, histology and microbiology.
Product Details
Product Name
Code
Specifiction
Crystal violet solution
G1014-50ML
50ml
Usage:
For suspension cultured cells, add crystal violet solution to cell suspension for 3-10 min. put 1 drop of the sample on a glass slide and smear it to form a homogeneous film. Allow the smear slide dry in air.
For adherent cells, wash the cells with PBS or saline solution twice, 1-2 min each. Stain the cells with crystal violet solution for 3-10 min. Then wash with PBS or saline solution adequately, dry in air.
Notes
Storage and transportation: Store at room temperature, kept in dark, valid for 12 months.
For your safety and health, please wear lab coats and disposable gloves for operation.
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