Lecithin Tween-80 Nutrient Agar

 PRODUCT:   Lecithin Tween-80 Nutrient Agar

Usages:

For the determination of total bacteria in cosmetics.

Principle:

Peptone and beef extract powder provides carbon and nitrogen sources, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium; Tween 80 can neutralize phenol, hexachlorophene, formalin, and ethanol can be a combination of both; agar as medium coagulant.

Formulation(per liter):

Peptone 20g

Beef extract powder 3g

Sodium chloride 5g

Agar 15g

Lecithin 1g

Tween 80   7g

Final pH7.3 ± 0.2

How to use:

1.Suspend 51g in 1 L of distilled water , stirring heated to boiling until completely dissolved, distribute into flask,autoclave at 121℃ for 15 minutes.

2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle

*Supplement:029226 TTC solution

Package:Packing material:Packed in plastic bottles with plastic film wrapped

                    Capacity:20 bottle/ carton

1.Offering Free Sample for Testing .

2.R & D team to provide technical support.

3.Provide packaging, labeling OEM customization.

Q1:

Sometimes color of dehydrated culture media  between batches have subtle differences,would this affect test results?

A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.

Q2:

After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?

A2: The reason may be:

The required hydration in the preparation process have not been done completely .That is to say   distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.

Q3:

Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?

A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 ℃ false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.