LSY-20033 Avian influenza virus rapid test kit AIV Ag rapid test

 Avian influenza virus antigen rapid test card

Catalog No. LSY-20033

1. Introduction

Avian influenza is caused by Orthomyxoviridae type A family specific virus. There are 3 types of

Influenza virus: A, B, C, as far as we know, only type A influenza virus can infect Poultry.

Avian influenza virus (AIV) antigen rapid test card is based on rapid immunochromatographic detection technology, to detect avian influenza virus antigen in birds’ tracheal and cloacal swabs (or feces). The sample to be tested is added to the sample hole, it will move along with colloidal gold-labeled anti-AIV Monoclonal antibody on the chromatography membrane. If there is AIV antigen in the sample, the antigen will combine with the antibody on test line, appear red wine color.

If there is not AIV antigen in the sample, no color appears.

This rapid test is based on double antibody sandwich, using colloidal gold immunochromatographic method, to detect avian influenza virus.

2. Contents

3. Test procedure

1) Use swab to collect sample from cloaca (recommended) or tracheal, insert the swab into cloaca or tracheal and wipe, rotate in the inner wall repeatedly. For young birds, it can use swab to collect fresh feces sample directly.

2) Insert the swab into the tube with sample buffer immediately, rotate forcefully for at least 10 times in the tube wall. Mix the solution evenly, make the sample been dissloved in the buffer fully. Press swab on the tube wall above the solution level, so the solution can be extruded at last, discard the swab.

3) If there are big particles of feces, be static for 1-2 min, make big particles precipitation.

4) Take out test card from sealed bag. Put it on flat surface, use dropper to draw upper bright liquid, drop 4 drops slowly, one by one, to the sample hole with “S” mark.

5) Read the result in 10-15 minutes, the result is invalid after 15 minutes.

4. Results judgement

1) Negative: only control line is seen wine red;

2) Positive: control line and test line are both seen wine red;

3) Invalidation: control line isn’t seen wine red.

5. Note

1) Do not use it if there is any damage to the Aluminum packed bag.Use it in 1 hour after open the package.

2) Test the collected sample immediately, avoid storage for a long time.

3) The collection of sample affect the testing accuracy, so ensure collecting sample correctly and fully.

4) Collecting feces sample too much will affect the moving of liquid and lead to wrong result (appear dark region), But too small sample may appear false negative result, so control the quantity of sample as following picture:

5) Avoid producing bubble when adding sample to the test card.

6) Deal with the used test card as local polluted material.

7) Do not use test card out of date.

6. Questions

1) Limitation of the product

This kit is a qualitative screening reagent, can detect AIV antigen accurately, but if sample is not enough or concentration of antigen is lower than sensitivity, it may appear negative result. A clear clinical diagnosis should be made after taking into account all clinical and laboratory phenomenon.

2) The sensitivity and specificity of the product.

The limit value to detect AIV Ag is 0.13 HAU, it has no cross-reaction with similar symptoms to those of fowl cholera, newcastle disease, infectious bronchitis

7. Storage and expiry date

Specifications: 40T/kit

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.

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