LSY-10034 Ochratoxine test ELISA Food safety rapid test kit

 Ochratoxin A (OTA) ELISA Test Kit

                       Catalog No.LSY-10034

1.  General

Ochratoxin A are secondary metabolites of Aspergillus and Penicillium strains, exist widely in grain and grain products, spices and coffee beans and other products. Ochratoxin A has a strong liver and kidney toxicity, and has teratogenic, mutagenic and carcinogenic effects, which is serious harmful to human health.

Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) has been a common method of Ochratoxin A detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of Ochratoxin A ELISA kit to analyze Ochratoxin A residue of samples is fast and accurate.

This kit is a new generation of fungal toxins detection products in application of ELISA technology, to minimize operating errors and work intensity.

2.  Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Ochratoxin A in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Ochratoxin A in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Ochratoxin A antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Ochratoxin A in it. This value is compared to the standard curve and the Ochratoxin A concentration is subsequently obtained.

3.  Application

This kit can detect Ochratoxin A in grain, feed and wheat flour samples qualitatively and quantitatively.

4.  Technical specifications

Sensitivity:   0.2 ppb

Detection limit:

Grain, feed…………………………………………………2.5ppb

Cross-reaction rate

Ochratoxin A …………………………………………………………100%

Ochratoxin B …………………………………………………………43%

Ochratoxin C ………………………………………………………….5%

5.  Components

1)  OTA Micro-well strips: 12 strips with 8 removable wells each

2)  OTA 5 x standards …………………………………2 ml each 

0ppb (zero standard), 0.2ppb, 0.5ppb, 2ppb, 4ppb

3)  OTA Enzyme conjugate ..........................................7 ml

4)  Substrate ................................................................. 12ml

5)  Stop solution............................................................. 10ml

6)  Concentrated wash buffer(10×)..........................50ml

7)  Sample extract reagent ............................................1 bottle

Storage and expiry date
Storage: store at 2 to 8 °C, not frozen.
Expiry date: 12 months; date of production is on box.

Our Mycotoxins Test kits 

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.

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