Zearalenone ELISA test kit
Catalog No. LSY-10030
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Zearalenone. The coupling antigen is pre-coated on the micro-well stripes. The Zearalenone in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Zearalenone antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Zearalenone in the sample. This value is compared to the standard curve and the Zearalenone residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min~15min
Detection limit:
Feed…………………………………………….............10ppb
Rice, Corn, Peanut………………………………………5ppb
Cross-reaction rate:
Zearalenone…............................................................100%
Recovery rate:
Feed, rice, Peanut, corn…………………………………100±30%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20X concentrated washing buffer | 40ml | white cap |
9 | 2X concentrated redissolving solution | 50ml | transparent cap |
4. Materials required but not provided
1) Equipment: ELISA Reader (450 nm/630nm), homogenizer, shaker, centrifuge, balance: 0.01g quantity sensitive, nitrogen-drying device, incubator, graduated pipettes, printer
2) Micropipettes: single-channel 20ml ~ 200ml, 100ml ~ 1000ml, multi-channel 30~300 μl
3) Reagents: Methanol, n-hexane.
Our Mycotoxins ELISA test kits
Product Code | Product Name | Spec | Sensitivity (ppb) | Sample Performance |
LSY-10028 | Aflatoxins B1 ELISA test kit | 96 well/kit | 0.02 | Feed, rice, corn, peanut, tissue, edible oil |
LSY-10032 | Aflatoxin M1 ELISA test kit | 96 well/kit | 0.03 | Milk, Milk powder, Yogurt |
LSY-10029 | Total Aflatoxins ELISA test kit | 96 well/kit | 0.02 | Feed, rice, corn, peanut, tissue, edible oil |
LSY-10030 | Zearalenone ELISA test kit | 96 well/kit | 0.1 | Feed, corn, peanut, rice |
LSY-10031 | Deoxynivalenol(DON) ELISA test kit | 96 well/kit | 2 | Feed, corn, peanut, rice |
LSY-10033 | Fumonisin B1 ELISA test kit | 96 well/kit | 0.5 | Feed, peanut, rice, maize |
LSY-10034 | Ochratoxin A ELISA test kit | 96 well/kit | 0.2 | Grain, feed |
LSY-10035 | Trichothecenes (T-2) ELISA test kit | 96 well/kit | 0.05 | Feed, peanut, rice |
Product Code | Product Name | Spec | Sensitivity (ppb) | Sample Performance |
LSY-20006 | Aflatoxins B1 rapid test strips | 50 test/kit 96 test/kit | 2 | Grain, feed, edible oil |
LSY-20007 | Total Aflatoxins rapid test strips | 50 test/kit 96 test/kit | 1 | Grain, feed, edible oil |
LSY-20038 | Aflatoxins M1 rapid test strips | 96 test/kit | 0.2-0.3 | Milk |
LSY-20078 | Ochratoxin A (OTA) rapid test strip | 40 test/kit | 5 | Grain, feed |
LSY-20084 | Deoxynivalenol(DON) rapid test strip | 50 test/kit | 50 | Grain, feed, edible oil |
LSY-20085 | Zearalenone(ZEN) rapid test strip | 50 test/kit | 10 | Grain, feed, edible oil |
LSY-20086 | Fumonisin rapid test strip | 40 test/kit | 200 | Grain and feed |
LSY-20087 | Trichothecenes (T-2) rapid test strip | 40 test/kit | 10 | Grain and feed |
We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.
Our advantages at the Diagnostic kits/ ELISA test kits field are as following:
* ISO9001:2008 certificated company
* Own a high-quality R&D team with many years of experience on bio-diagnostic field.
* Professional manufacturer of ELISA test kits, offer OEM service.
*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.
*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.
ISO9001:2008 Certificate