(1)Return the unopened detection card and sample solution to room temperature before detecting;
(2)Take out the detection card and enzyme marker holes from the original aluminum foil bag and use it within half an hour;
(3)Use pipette to absorb 80 μL of sample solution into the enzyme-labeled well, blow repeatedly until the red substance in the well is completely dissolved, wait for 2 minutes;
(4)Lay the detection card flat, drop all the solution in the suction hole into the sample well, and start the timing after the sample is added;
(5)Judged according to the schematic diagram after 5 minutes for reaction, it will invalid in other time.
Negative results: T line is deeper than C line, or the same depth, indicating that the concentration of furacillin metabolite in the sample is below its detection limit;
Positive results: T line is shallower than C line, or T line is green, indicating that the concentration of furacillin metabolite in the sample is higher than its detection limit;
Ineffective reaction: if the test result C line does not appear, the reagent has failed.