High accuracy Medica Home and Hospital Use Dengue NS1/IgG/IgM Antigen Combo Rapid Antibody All Diagnostic Test Kit Cassette Type

Dengue NS1 -IgM -IgG Rapid Test Cassette (Serum/Plasma/ Whole Blood)

 INTRODUCTION

Dengue viruses,transmitted by the mosquito Aedes aegyptiand Aedes albopictus mosquitoes. are widely distributed throughout the tropical and Subtropical areas of the world. There are four known distinct serotypes(dengue virus 1, 2. 3 and 4), In children infection is often subclinical or causes a self-limited febrile disease, However, if the patient is infected second times with a different serotype, a moresevere disease. dengue hemorrhagic fever or dengue shock syndrome,is morelikely to occur.Dengue is considered to be the most important arthropod-borne viral disease due to the human morbidity and mortality it causes, NS1 is a highly-conserved glycoprotein that is present at high concentrations in the sera of dengue-infected patients during the early  clinical phase of the disease.NSl antigen is found from the first day and up to 9 days after onset of fever in sample of primary or secondary dengue infected patients.Usually IgM does not become detectable until 5 to 10 days after the onset of illness in cases of primary dengue infection and until 4 to 5 days aftef onset of illness in secondary infections. In primary infections,IgG appear the 14th day and persist for life, secondary infections show that IgG rise within 1-2 days alter the onset of symptoms and induce IgM response altef 20 days of infection.

Dengue NS1-IgM-IgG test cassette is a lateral flow chromatographic immunoassay for the simultaneous detection and differentiation of NS1,IgM and IgG in human serum (or plasma). The kit is a diagnostic device for qualitative detection .

TEST PROCEDURE

1.Open a pouch containing a cassette, remove the test kit from the pouch and place it

horizontally on the desk.

2.Pipette one drop(5µl) of specimen into the same well and then add one drop(50µl) of diluent into the same well.

3.Read results within 15 minutes. Do not read results after 20 minutes.

INTERPRETATION OF RESULTS

Negative: Only control pink band appears on test region of the cassette. This indicates that there is no determinand in the specimen.

Positive: Two pink bands(C,T) appear on test region of the cassette. This indicates that the specimen contains detectable amount of determinand.

Invalid: If without colored band appears at control region, this is an indication of a possible error in performing the test. The test should be repeated using a new one.