Genomic DNA Isolation Extraction Kit for Paraffin-Embedded Tissue

Genomic DNA Isolation Kit (Paraffin-embedded tissue)

Cat No. SN027-0100 Size: 100 Reactions
Cat No. SN027-0004 Size: 4 Reactions

Sample: 25 mg of paraffin-embedded tissue
Format: spin column
Column capacity: up to 50 μg
Operation time: within 60 minutes

Description
The Genomic DNA Isolation Kit (Paraffin-embedded tissue) is designed specifically for genomic DNA isolation from animal tissue samples. This unique buffer system ensures total DNA with high yield and good quality from samples. The spin column system is designed and purified or concentrate DNA products which have been previously isolated using buffers. The entire procedure can be completed in one hour without phenol / chloroform extraction. The isolated DNA is suitable for PCR or other enzymatic reactions.

Features
1. Ready-to-use genomic DNA for high performance in any downstream application.
2. Optimized lysis buffer for the efficient lysis.
3. Designed to rapidly purify high-quality DNA using spin column format.

Applications

1. Gene cloning.

2. Southern blotting.

3. PCR.

4. SNP genotyping.

Quality Control
The quality of the Genomic DNA Isolation Kit (Paraffin-embedded tissue) is tested on a lot-to-lot basis to ensure consistent product quality.

Required Materials
- Mortar and pestle

- Water bath/ Dry bath
- Proteinase K (10 mg/ml)

- Isopropanol
- Absolute ethanol

- Microcentrifuge tubes

- Micropestle

- Xylene
- RNase A (50 mg/ml) (Optional)

Buffer Preparation
TE buffer, pH8.0 (Selective): 10 mM Tris-HCl, pH 8.0 with 1 mM EDTA