Description:
Animal Total RNA Isolation kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency.It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, simple and time-saving; RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula Lots of samples.
The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.
Specifications:
50 Preps, 200 Preps
Product components:
Buffer RL1 , Buffer RL2 |
Buffer RW1, Buffer RW2 |
RNase-Free ddH2O, DNA-Cleaning Column |
RNA-Only Column |
Features&advantages:
-No need to worry about RNA degradation; the whole system is RNase-Free
-Effectively remove DNA-using DNA-Cleaning Column
-Remove DNA without adding DNase
-Simple-all operations are completed at room temperature
-Fast -operation can be completed in 30 minutes
-Safe-no organic reagent extraction required
-High purity -OD260/280≈1.8-2.1
Kit application:
It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.
Product Parameters:
-Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
-Samples: animal tissues, cultured cells
-Dosage: Tissues 10-20mg, Cells(2-5)×106
-Maximum DNA binding capacity of purification column: 80 μg
-Elution volume: 50-200 μl
Work Flow:
Diagram:
Storage and Shelf life:
The kit can be stored for 24 months at room temperature (15–25 ℃) and 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).
Foregene Co., Ltd is specialtized in the IVD kits and R&D reagents production and services, which is integrated in R&D, production and marketing. Foregene has the world’s leading Direct PCR platform.The main products include various IVD kits, nucleic acid purification reagents,PCR and Direct PCR reagents,Genotyping kits, etc. The technology and product performance indicators used are all leading the world status,widely applied in hospitals, institutions, medical laboratories, etc.
Foregene Co., Ltd. was founded in April 2011 by well-known scholars who returned from studying at Yale University, famous inventors and senior industry professionals.Foregene’s R&D team consists of 40 people(mainly phD and master), has 2500 m² comprehensive building, 600 m² P2 laboratory, and 1200m² GMP production facility.
With Direct PCR technology, there’s no need for cumbersome nucleic acid purification, the sample is directly added to the system and then on the machine.Now all indicators of Foregene’s Direct PCR technology meet the needs of clinical diagnosis.