Diagnostic elisa test kit IgM Antibody to Hepatitis A Virus HAV

1. Anti-μ-chain Coated Micro well Plate

2. Enzyme Conjugant (HAV-Ag-HRP)

3. Conjugate HAV-Ag

4. Negative Control Serum

5. Positive Control Serum

6. 20 X Wash Buffer (dilution prior to use)

7. Substrate A

8. Substrate B

9. Stop Solution(2M H₂SO₄)

10. Plastic Bag

11. Seal Paper

12. Manual

ELISA Kit for Antibody IgM to Hepatitis A Virus(HAV)

CatalogNo. NAME PACKAGE SIZE:

BE301A ELISA Kit for Antibody IgM to Hepatitis A Virus (HAV) 96 tests/Kit

 SUMMARY

The Hepatitis A virus occurs through ingestion of fecally contaminated materials from an HAV positive source - another person, contaminated food, or contaminated water.HAV is responsible for 20-65% of all cases of acute hepatitis reported each year in adults.

The specific routine diagnosis of acute hepatitis A is made by finding anti-HAV IgM in the serum of patients. A second option is the detection of virus and/or antigen in the faeces.

Hepatitis A IgM is the first antibody produced by the body when it is exposed to hepatitis A, and indicate a recent infection with hepatitis A virus. IgM anti-HAV antibodies generally can be detected in the blood as early as 2 weeks after the initial HAV infection. In acute infection period, anti-HAV IgM is present of high level and the titer of it may be up to 1:10000. It is decreased significantly and the titer is low to 1:100. The IgM antibody even disappeared 12 months after the infection, and IgG is present instead.IgG anti-HAV antibodies mean that you have had a hepatitis A viral infection. About 8 to 12 weeks after the initial infection with hepatitis A virus, IgG anti-HAV antibodies appear and remain in the blood for lifelong protection (immunity) against HAV.

The hepatitis A IgM test is used to screen for early detection of infection and is used to diagnose the disease in a patients with evidence of acute hepatitis

PRINCIPLE

This kit is a solid-phase enzyme immunoassay based on an captured ELISA principle. ELISA method to detect anti-HAV IgM. The purified anti human IgM monoclonal antibody (Anti-μ-chain) is coated on the solid phase of multi-wells. Serum sample, HAV-Ag and Horseradish peroxidase labeled HAV-Ag are added to coated wells. If HAV-IgM is present in the sample, a complex of Anti-μ-chain-HAV-IgM -HAVAg labeled with HRP will form. Wash wells to remove other unbounded serum components, incubate with substrate (TMB) to form a colored product, and measure the absorbance at 450nm to indicate the presence or absence of HAV-IgM in the sample. The test is special, sensitive, reproducible and easy to operate.

CHARACTERISTIC

1. Using capture ELISA.
2. Provide a vial of heat-inactivated sample, diluted to a titer of 1:100 0 for antibody IgM to HAV.