Blood Genomic DNA Extraction Kit (Spin column) /spin column dna
Tissue Blood Genomic DNA Extraction Kit DNA Purification Kit
Description
Uses the silica-gel-membrane technology isolate Genomic DNA without phenol/chloroform.
Homogenization is not necessary, directly lysed by Proteinase K.
Optimized to allow selective binding of DNA to the silica-gel membrane.
The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites.
Pure DNA is then eluted in water or low-salt buffer, ready to use.
Suitable for anticoagulated blood. 400 μl whole blood yield 3-10 μg high pure genomic DNA. The purified DNA can be used in common downstream applications such as sequencing, restriction digestion,etc.
Feature.
High Efficient – 3-10 ug genomic DNA from 400 ul whole blood
Safe - no phenol/cholroform extraction step.
High purity - Purified DNA is ready for downstream application such as PCR, restriction digestion.
Applications
Purified DNA is free from contaminants and enzyme inhibitors, and typically has A260/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:
Restriction digestion
PCR
Labeling
Southern hybridisation
Library construction
Store
store Protein K at -20°C
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