Aflatoxin M1 ELISA Test Kit

Intended Use
The Aflatoxin M1 ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Aflatoxin M1 concentrations in liquid milk, fermented milk, milk power, cheese, etc.
Use Principles
The Aflatoxin M1 kit is an indirect competitive enzyme-labeled immunoassay. The Aflatoxin M1 antigen is precoated on the wells. The aflatoxin M1 in the sample compete the aflatoxin M1 antibody (antibody solution) with aflatoxin M1 antigen on the well, while the M1 antibody combines with the enzyme conjugate. Then pipe the substrate solution to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Aflatoxin M1 concentration of the samples is derived.
Cross Reactivity
AFM1..................................................................100%
AFM2..................................................................15.5%

1. Take out the reagents from the refrigerator and allow them to reach room temperature (20-25℃) prior to running the test.
2. Place the appropriate number of test wells into a microwell holder. Be sure to re-seal unused wells in the zip-lock bag with desiccant stored at the temperature 2-8℃ (do not freeze).
3. Number:Number the microwells for the standard and samples separately. Running standard and samples in duplicate.
4. Using a pipette with disposable tips, add 50μL of standard or samples to the appropriate test wells. Be sure to use a clean pipet tip for each. Then dispense 50µL of Enzyme Conjugate into each test well. At last, dispense 50µL of antibody Solution into each test well.
5. Shake the plate gently to mix contents, cover the plate rack and incubate the test wells at 25℃ keeping away from light for 30 minutes.
6. Open the cover on the plate and dump the contents of the wells into an appropriate waste container. Fill the wells to overflowing with wash solution and dump (250µL each well). Repeat for a total of four to five washes (wait 10s between each wash).
7. Following the last wash, tap the inverted wells onto absorbent paper to remove the last of the wash solution.
8. Dispense 50µL Substrate A and 50µL Substrate B into each well in order.

Meizheng Group is located in Zhongguancun Science park of Yanqing,Beijing,Since 2007,The group and its subsidiaries have been developing rapid testing solutions for the food safety.
Meizheng Group is committed to providing customers with high quality services,efficient and continuous improvement diagnotis products,protecting our food from farm to fork.
Our products include rapid test kits and instruments for detection of Mycotoxin,antibiotics,food addictive residues,pesticides and,serving clients all over the world in fields like dairy,meat&seafood,feed,grain and oil,food processing etc.
Nowadays,the products of Meizheng Group are sold well both domestic and abroad like Russia,France,Taiwan,The United States,India,Vietam and other countries/Regions all over the world.