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Anti-streptolysin - O (ASO) Latex Slide agglutination Test Kit

ASO KIT 

METHOD PRINCIPLE
ASO Latex slide test for detection of antibodies to streptolysis O is based on the principle of agglutination. The test specimen (serum) is mixed with ASO latex reagent and allowed to react. If antibodies to streptolysin O are present then a visible agglutination is observed. If antibodies to streptolysin O are not present or are in concentration less than 200 IU/ml then no agglutination will be observed.

1. Store the reagent at 2-8°C. DO NOT FREEZE

2. The Shelf life the reagent is as per the Expiry date mentioned on the reagents vial labels

SPECIMEN

Only Serum should eb used to testing. Should be a delay in testing occur, store the samples at 2- 8°C. Samples can be stored for upto a week. Do not use hemolysed serum

PROCEDURE
QUALITATIVE METHOD
1. Pipette one drop of test sample onto the glass slide using a disposable pipette provided with the kit.
2. Add one drop of ASO latex reagent to the drop of test sample on the slide.
3. Using a mixing stick, mix the serum and the ASO latex reagent uniformly over the entire circle. Do not let the dropper tip touch the liquid on the slide.
4. Immediately start a stopwatch. Rock the slide gently, back and forth, observing for agglutination macroscopically at two minutes. Proceed similarly with each dilution as test specimen
5. Do not read the results beyond 2 minutes.

SEMI QUANTITATIVE METHOD
1. Using isotonic saline prepare serial dilutions of the serum sample positive in the qualitative method 1:2,1:4,1:8,1:16 and so on.
2. Pipette the diluted specimens onto the slide. Start with the 1:2 diluted test specimen.
3. Add a drop of ASO reagent to it. Mix well. Spread the mixture uniformly over the entire circle.
4. Immediately start a stop watch. Rock the slide gently, back and forth, observing for agglutination macroscopically at two minutes. Proceed similarly with each dilution as test specimen.

INTERPRETATION OF TEST RESULTS
Qualitative Method
Agglutination is a positive test result and indicates the presence of detectable levels of Anti-Streptolysin O in the test specimen. No agglutination is a negative test result and indicates the absence of detectable levels of Anti- streptolysis O in the test specimen.

Semi Quantitative method

Agglutination in the highest serum dilution corresponds to the amount of ASO in IU/ml present in the test specimen.

Calculation

ASO (IU/ml) = S x D

Where S = Sensitivity of the reagent ie., 200 IU/ml

            D = Highest dilution of serum showing agglutination.

NOTES:
1. In vitro diagnostic reagent for laboratory and professional use only. Not for medicinal use.
2. All the reagents derived form human source have been tested for HBsAg and anti HIV antibodies and are found to be non-reactive. However handle the material as if infectious.