Myson cellulose nitrate transfer membranes are unsupported (Type SCN) or supported (Type SCNR) membranes optimal for transfer of both proteins & nucleic acids. Cellulose nitrate Membranes is a proven medium for western, northern, and southern blotting that produces excellent signal to noise result. MICROPORE Cellulose nitrate transfer membranes are easy to handle, wets readily with water –based solutions, and resists the brittleness usually associated with cellulose nitrate .The 0.45 µm cellulose nitrate pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers.
Internal support gives better handling.
TECHNICAL SPECIFICATIONS |
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MOC CN Membrane Filters |
Pure CN polymeric membrane filters with or without internally reinforced support. |
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MOC M-funnels and E-Funnels |
Polypropylene top, bottom 7 Plug Assembly with Acrylic Lid |
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Pore size |
0.2 µm |
0.45 µm |
Integrity test by Bubble point method (DI-Water wet) |
52-58 psi 3.66-4.07 Kg/cm2 |
32-38 psi 2.25-2.67 Kg/cm2 |
Minimum Water flow rate (ml/min/cm2) At L'.P = 10 Psi ,25 °C |
20 |
45 |
Size/diameter (mm) |
82mm, 92mm, 142mm, 150mm, 200mmx200mm, 300mmx300mm, 300mmx3m |
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SALIENT FEATURES OF SCN MEMBRANES: |
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Sterilization |
Autoclavable at 121oC up to 5 cycles |
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Maximum Operating Temperature |
80oC Continuous
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Maximum Operating Pressure |
5Kg/cm2 |
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Biosafety |
Passes Biological test for class VI Plastics are describing as USP |
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Oxidizable Matter |
Passes as per USP |
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Extractables With DI-Water |
Less than as Specified in USP |
Benefits:
· Compatible with all standard immune-blotting and nucleic acid detention methods.
· Provides superior binding capacity without background interference.
· Pore size produces excellent signal-to-noise blotting results.
· Uniform pore size distribution.
Applications:
· Transfer of protein from SDS-PAGE gels to the membrane in preparation for western blotting
· Transfer of a wide range of protein molecular weights and nucleic acids >500 bp.
· Protein blotting.
· Colony Plague lifts and Enzyme Immunoassays.